Ion Research Panels:

Panel Name Research Area Genome Number of amplicons per pool
(# of tubes)
Tags Actions
Ion AmpliSeq™ Transcriptome Human Gene Expression Research Panel
A26325
Gene Expression Research hg19 AmpliSeq Transcriptome ERCC v1 Pool 1: 20802 amplicons
(1 tube)
RNA
Ready-to-Use Panel
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RNA Transcriptome Ready-to-Use Design Details

The ready-to-use design details are available for download. In a compressed folder, there are a number of files with diverse information. Although the type of data in all of the files is plain text and they can be opened using any text editor, we recommend using a spreadsheet such as MS Excel for viewing their contents given their multicolumn format.

Summary: The Ion AmpliSeq™ Transcriptome Human Gene Expression Research Panel is used to measure expression levels of over 20,800 RefSeq genes using only 1 pool. The panel targets 18,574 coding genes and 2,228 non-coding genes based on UCSC hg19 annotation. This panel contains 20,802 amplicons of approximately 150 bases in length, in a single pool.

The Ion AmpliSeq™ Transcriptome Human Gene Expression Research Panel is a revolutionary offering allowing for the simultaneous measurement of the expression levels of over 20,800 human RefSeq genes in a single pool. The panel targets 18,574 coding genes and 2,228 non-coding genes based on UCSC hg19 annotation. We recommend sequencing 8 samples on a Proton PI™ Chip.

  • Sensitive measurement of gene expression fold changed between samples
  • Amplicons designed to be most inclusive for transcripts of the target gene
  • Requires only 10 ng of FFPE total RNA (no rRNA depletion or polyA+ selection required)
  • Create libraries using the Ion AmpliSeq™ Kit Plus reagents and Ion Xpress™ Barcode Adapters
  • Compatible with Ion Chef™ or Ion OneTouch™ Systems for template prep and the Ion Proton™ or Ion PGM™ Systems for sequencing

This panel contains 20,802 amplicons (41,604 primers), approximately 150 bases in length, in a single pool.