Ion AmpliSeq™ TB Research Panel  Hide Panel Description

Tuberculosis (TB) remains one of the main infectious diseases affecting developing countries. According to WHO, globally in 2013 there were 9 million people who fell ill with TB. Furthermore, an estimated 480,000 people developed multidrug-resistant TB (MDR-TB), and there were an estimated 210,000 deaths from MDR-TB. The TB Research Panel has been developed in collaboration with leading public health institutes. The panel assesses 8 genes related to antimicrobial resistance (embB, eis, gyrA, inhA, katG, pncA, rpoB, rpsL). For further details see ASM 2015 poster “Strain-Typing and Antibiotic Resistance Profiling From Research Samples Using Highly Multiplexed Targeted Library Construction with High Throughput Semi-Conductor Based Sequencing” J. Bishop et al.

Design Date Dec 2013 Publication: Bishop et al. Poster session presented at 115th General Meeting of the American Society for Microbiology; 2015 May 30- June 2; New Orleans, LA. (Poster link)
Author: John Bishop (john.bishop@thermofisher.com)
Affiliation: Ion Torrent, Thermo Fisher Scientific
Recommended Application Identification of gene variants correlating with antimicrobial resistance in Mycobacterium tuberculosis Recommended Configuration Sample per Chip: 36 per 318 chip
Minimum coverage: 1000x
Sample Type TB culture sample
Number of sample in Publication N/A Observed Performance Panel uniformity: >90 %
Reads on-targets: >90 %
Input DNA required 10 to 100 ng per library
Disease Research Area: Infectious Disease/ TB
  • Chip Calculator
  • 2 (20 ng)
  • Pools (Input DNA) Multiple Pools
  • Pool1: 55 amplicons | Pool2: 54 amplicons
  • 14.91 kb
  • Panel Size