Ion AmpliSeq™ Hematology Research Panel  Hide Panel Description

Research to identify a specific genetic cause of hematological disorders is important but can be difficult because of pleiotropy, locus heterogeneity and accessibility of gene tests. The panel was developed as a quick, accurate and cost effective method to identify genetic mutations associated with inherited hematological disorders. The Hematology panel assesses 394 genes known to harbor mutations affecting the blood. In addition to targeting all coding regions, an additional 161 non-coding disease-causing loci are targeted.

Design Date August 2013 Avg Amplicon Length 253 bp Insert Size 205 bp
Recommended Application Germ line mutation detection Recommended Configuration Sample per Chip: 2 per 318 chip
Average coverage: 445x
Sample Type Standard
Design Statistics Number of genes in panel: 394
Average gene coverage: 99.5%
Non-coding hotspots: 161
Observed Performance Uniformity of coverage: 93.8%
% reads on target: 97%
Positive predictive value: 99.5%
Input DNA required 10ng per tube
2 pool
20 ng total
Disease Research Area: Hematological Disorders
  • Chip Calculator
  • 2 (20 ng)
  • Pools (Input DNA) Multiple Pools
  • Pool1: 3722 amplicons | Pool2: 3712 amplicons
  • 826.24 kb
  • Panel Size