Ion AmpliSeq™ Designer performance specifications for custom DNA panels
|
Specification |
Value |
|---|---|
|
Input DNA amount per primer pool |
As little as 1 ng |
|
Available reference genomes |
Cow, chicken, chinese hamster, dog, human, maize, mouse, pig, rice, sheep, soybean, tomato |
|
Custom references |
Upload any reference genome to a password-protected cloud space |
|
Maximum amplicon length options |
140 bp (optimized for cfDNA samples) 175 bp (optimized for FFPE samples) 275 bp or 375 bp (standard samples) |
|
Primer pool size |
12 to 6,144 primer pairs per pool, targeting a single gene or thousands of genes |
|
Primer pairs per pool |
12 to 24,000 |
|
Input DNA required |
As little as 1 ng of input DNA required per primer pool |
|
Target size |
1 kb to 5 Mb |
|
Sample multiplexing |
Up to 384 barcodes available |
|
Pools per panel |
Typically 1 to 2 pools |
|
Panels with ≤96 primer pairs |
2,250 reactions (750 prepooled and multiplexed)[1] |
|
Panels with >96 primer pairs |
9,000 reactions (3,000 prepooled and multiplexed)1 |
|
Target design rate Target design rate-bases covered by the insert region of amplicons out of total target bases submitted |
>90% |
|
Coverage uniformity Typical coverage uniformity = bases covered at >20% of the mean coverage. |
>85% |